Pre-clinical studies
In vitro: the cyto-toxicity of Bleomycin or Cysplatin is increased by 1.000 to 10.000 times when the cultures are subject to electroporation (1-3).
In vivo: the growth control of mice implanted tumours was significantly higher when treatment with Bleomycin or Cysplatin was associated with electroporation (4- 6).
The efficacy of ElectroChemoTherapy in controlling the growth of tumoral lesion is documented by several pubblications.
References
1.Pucihar G, Mir LM, Miklavcic D: The effect of pulse repetition frequency on the uptake into electropermeabilized cells in vitro with possible applications in electrochemotherapy. Bioelectrochemistry 57, 167- 172, 2002.
2.Mir LM, Banoun H, and Paoletti C: Introduction of definite amounts of non-permeant molecules into living cells after electropermeabilization: direct access to the cytosol; Exp Cell Res. 175: 15-25, 1988.
3.Gehl J, Skovsgaard T and Mir L M: Enhancement of cytotoxicity by electropermeabilization: an improved method for screening drugs. Anti-Cancer Drugs, 9, 319-325, 1998
4.Gehl J and Mir LM: Determination of Optimal Parameters for in Vivo Gene Transfer by Electroporation, Using a Rapid in Vivo Test for Cell Permeabilization, Biochemical and Biophysical Research Communications 261, 377-380,1999.
5.Gehl J, Soerensen T H, Nielsen K, Raskmark P, Nielsen S, Skovsgaard T, Mir L M: In vivo electroporation of skeletal muscle: threshold, efficacy and relation to electric field distribution. Biochimica et Biophysica Acta 1428, 233-240, 1999
6.Mir LM: Therapeutic perspectives of in vivo cell electro-permeabilization. Bioelectrochemistry 53, 1-10, 2000. |
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